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Monday, July 1, 2013

AP Biology Enzyme Synthesis Lab Report

Enzyme Catalysis         Introduction and Overview- In this lab, we will observe the reincarnation of enthalpy peroxide (H2O2) into peeing and atomic number 8 gas. An enzyme known as catalase facilitates this conversion response. The catalase enzyme acts as catalysis, back up lower the energy needful to activate the chemical reaction plot of land the enzyme itself is not affected. The reaction could sign on place without the help of catalase, unless it would happen a indorse more(prenominal) slowly because more energy is needed for the reaction.         The inwardness being acted on by the enzyme is known as the substratum, and in this case is hydrogen peroxide. The enzyme changes these subst esteems into products, in this case water and O2 gas. The beggarly of this lab is to observe and tax the judges of conversion of hydrogen peroxide to water and oxygen by the enzyme catalase over diametrical periods of time.         To footprint the rate of the reaction, we measure the rate of disappearance of the substratum of over time. Potassium permanganate (KMn04) is employ to indicate the presence of severally remain H2O2 in the rootage aft(prenominal) the reaction. The summate of stay substrate is an indication of how oft the conversion reaction took place.         after allowing an enzyme to act on a substrate solution for sundry(a) periods of time and measuring the amount of remaining substrate, a rate of reaction can be calculated. And that is what the experiment boils down to, tally out the rate of an enzyme catalyzed reaction.
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I predict that the rate for reaction will remain moderately constant over time.         Materials- · buret         Â· 2% KmnO4                  Â· 1M H2SO4 (Sulfuric acid)         Â· H2O (Water)         Â· tater         Â· 1.5% H2O2 (Hydrogen Peroxide)         Â· Catalase         Â· 1 ml spray                  Â· 10 ml spray         Â· Stopwatch         Â· Six 50 ml beakers         Â· 2 more... If you essential to get a full essay, order it on our website: Ordercustompaper.com

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